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. 2020 Feb 20;10(4):e3530. doi: 10.21769/BioProtoc.3530

Table 2. Recipe of Digestion buffer B.

Component Final concentration Stock concentration* Volume for 50 ml
Deoxyribonuclease I 100 μg/ml Stock as powder 5 mg
Dispase II 5 mg/ml Stock as powder 250mg
HEPES 20 mM 1M 1 ml
DMEM/F12 medium - - Up to 50 ml#