Figure 2.

Effects of l-theanine pre-exposure on affective and sensorimotor gating impairments induced by adolescent THC exposure. A, Schematic representation of light–dark box anxiety test. B, C, Adolescent THC-treated rats spent more time in the dark (B) and re-emerged later (C), indicating increased anxiety levels. l-Theanine administration partially prevents long-term THC-induced anxiety (vehicle, n = 10; THC, n = 12; thea + THC, n = 11; theanine, n = 8; two-way ANOVA, post hoc Fisher's LSD; *p < 0.05, **p < 0.01). D, Schematic representation of PPI apparatus setup. E, Average startle amplitude response was not affected by any treatment. F, Compared with vehicle-treated controls, THC-treated rats exhibited impairments in sensory motor gating, which were fully prevented by l-theanine (vehicle, n = 8; THC, n = 11; thea + THC, n = 11; theanine, n = 8; two-way repeated-measures ANOVA, post hoc Fisher's LSD; *p < 0.05, **p < 0.01, ***p < 0.001).