Table 3.
Ih genetic manipulations disrupt circadian locomotor activity organization
| Genotype | DD analysis |
LD analysis |
||||||
|---|---|---|---|---|---|---|---|---|
| Tau (h) | Rhythm (%) | n | N | MAI | EAI | n | N | |
| pdf,dicer/+ | 24.00 ± 0.07 | 95 ± 3 | 75 | 3 | 0.73 ± 0.02 | 0.88 ±0.02 | 55 | 2 |
| UAS-IhRNAi/+ | 23.71 ± 0.02 | 92 ± 6 | 39 | 0.77 ± 0.03 | 0.80 ± 0.03 | 21 | ||
| pdf,dicer>UAS-IhRNAi | 23.95 ± 0.03 | 74 ± 8* | 40 | 0.67 ± 0.03 | 0.80 ± 0.02 | 23 | ||
| pdfGS/+, RU | 24.59 ± 0.41 | 97 ± 2 | 64 | 3 | 0.64 ± 0.01 | 0.66 ± 0.02* | 76 | 3 |
| UAS-IhRNAi/+, RU | 23.75 ± 0.06 | 100 ± 0 | 41 | 0.72 ± 0.02 | 0.58 ± 0.01 | 50 | ||
| pdfGS>UAS-IhRNAi, RU | 24.81 ± 0.75 | 57 ± 12* | 67 | 0.59 ± 0.01* | 0.60 ± 0.01 | 74 | ||
| control | 24.02 ± 0.05 | 96 ± 2 | 86 | 4 | 0.68 ± 0.03 | 0.84 ± 0.02 | 62 | 2 |
| Ihf01485/+ | 23.87 ± 0.03 | 96 ± 3 | 80 | 0.71 ± 0.04 | 0.89 ± 0.02 | 32 | ||
| Ihf01485 | 23.56 ± 0.05 | 60 ± 3* | 98 | 0.55 ± 0.05 | 0.65 ± 0.03* | 34 | ||
| Ihf03355/+ | 23.86 ± 0.05 | 99 ± 1 | 96 | 0.73 ± 0.03 | 0.92 ± 0.02 | 30 | ||
| Ihf03355 | 23.88 ± 0.08 | 39 ± 12* | 104 | 0.51 ± 0.03* | 0.67 ± 0.03* | 30 | ||
DD analysis (left): the average free running period and percentage of rhythmicity of populations of male flies of the indicated genotypes are shown. Values represent the average of N independent experiments ± SEM, n indicates total number of individuals tested; * indicates statistically significant difference (p < 0.05) after a one-way ANOVA comparing experimental genotypes to genetic controls. UAS-IhRNAi refers to the genetic combination of two UAS-IhRNAi constructs: DRSC 29574 + VDRC KK 110274). In the case of the Ih null mutants, Ihf01485 and Ihf03355, homozygotes were compared with a w1118 control and to heterozygotes (Ihf03355 crossed by w1118). RU refers to the presence of the steroid RU486 (200 μg/ml), the activator of the GS system, in the food media. LD analysis (right): morning anticipation index (MAI) and evening anticipation index (EAI) were calculated for the same genotypes. *Indicates statistically significant difference (p < 0.05) after Kruskal–Wallis statistical analysis with Dunn's multiple comparisons test. Significant differences (p – 0.05) compared with genetic controls are displayed in boldface.