. 2021 Jan 27;41(4):689–710. doi: 10.1523/JNEUROSCI.2322-20.2020

Table 5.

Sleep parameters after genetic manipulation ofI_h

Genotype	Total sleep (min)	Daytime sleep (min)	Nighttime sleep (min)	Bout duration (min)	Sleep bout number	Latency lights on (min)	Latency lights off (min)	Activity index	n	N	Temperature (°C)
I_h^f⁰³³⁵⁵	1026.6 ± 17.8^a	430.0 ± 14.3^a	599.9 ± 8.4^a	17.6 ± 0.6^b	58.5 ± 1.8^a	25.0 ± 5.8^b	15.3 ± 1.9^b	4.24 ± 0.18^a	56	3	25
I_h^f⁰³³⁵⁵/+	956.9 ± 21.6^a,b	394.6 ± 11.8^a	560.5 ± 15.0^a,b	29.0 ± 1.6^a	36.4 ± 1.7^b	71.5 ± 6.3^a	27.1 ± 3.7^a	2.18 ± 0.08^b	69
w¹¹¹⁸	922.2 ± 20.8^b	380.5 ± 12.6^a	533.4 ± 17.4^b	28.4 ± 1.7^a	35.3 ± 1.6^b	78.7 ± 7.3^a	27.6 ± 3.0^a	2.02 ± 0.05^b	66
c929-Gal4;tub-Gal80^TS>UAS-I_h^RNAⁱ	921.7 ± 31.6^a	464.1 ± 16.1^a	457.7 ± 21.6^a	17.0 ± 0.9^a	57.5 ± 1.9^a	18.1 ± 3.1^a	15.9 ± 2.7^b	2.16 ± 0.07^b	58	2	30
c929-Gal4;tub-Gal80^TS/+	773.0 ± 50.7^b	432.6 ± 25.5^a	340.4 ± 30.3^b	15.7 ± 0.9^a	49.4 ± 1.8^b	51.0 ± 12.0^a	45.7 ± 8.1^a	1.88 ± 0.05^c	31
UAS-I_h^RNAⁱ/+	793.5 ± 38.1^b	424.8 ± 21.5^a	368.7 ± 21.1^b	16.0 ± 0.8^a	50.6 ± 1.4^b	25.5 ± 7.5^a	17.4 ± 2.9^b	2.52 ± 0.09^a	61
R6-Gal4;tub-Gal80^TS>UAS-I_h^RNAⁱ	1264.9 ± 14.2^a	636.7 ± 7.6^a	630.7 ± 21.6^a	37.1 ± 2.5^a	41.7 ± 2.0^c	2.2 ± 0.7^c	16.8 ± 3.7^b	2.63 ± 0.08^a	85	3	30
R6-Gal4;tub-Gal80^TS/+	1195.6 ± 19.6^b	605.9 ± 10.8^b	589.6 ± 30.3^b	31.2 ± 3.1^b	47.3 ± 1.9^b	5.4 ± 1.1^b	24.7 ± 6.2^b	2.16 ± 0.06^b	87
UAS-I_h^RNAⁱ/+	1015.7 ± 23.7^c	515.0 ± 12.8^c	504.6 ± 21.1^c	20.5 ± 0.8^c	52.5 ± 1.2^a	13.6 ± 2.8^a	18.2 ± 2.4^a	2.21 ± 0.08^b	89
pdf-Gal4, UAS-dicer2;tub-Gal80^TS> UAS-I_h^RNAⁱ	955.9 ± 27.8^a	603.5 ± 12.3^ab	357.7 ± 20.5^a	25.8 ± 1.1^b	39.2 ± 1.6^a	17.2 ± 3.3^b	19.4 ± 4.6^b	1.82 ± 0.03^a	61	2	30
pdf-Gal4, UAS-dicer2;tub-Gal80^TS/+	847.9 ± 15.7^b	632.4 ± 7.4^a	215.5 ± 12.5^c	30.1 ± 1.0^a	30.0 ± 1.8^b	22.5 ± 1.7^a	36.5 ± 5.6^a	1.64 ± 0.03^b	63
UAS-I_h^RNAⁱ/+	848.7 ± 20.0^b	573.1 ± 12.9^b	274.9 ± 15.6^b	22.4 ± 1.1^c	41.9 ± 1.7^a	14.8 ± 1.9^b	24.5 ± 3.8^a	1.81 ± 0.03^a	61
c929-Gal4>UAS-I_h^RNAⁱ	980.5 ± 29.9^a	496.1 ± 15.0^a	484.1 ± 17.2^a	18.2 ± 1.3^a	60.3 ± 2.1^a	8.6 ± 2.4^c	21.7 ± 2.6^b	1.98 ± 0.04^a	72	3	25
c929-Gal4/+	793.1 ± 26.8^b	394.4 ± 13.5^c	401.4 ± 17.1^b	15.5 ± 0.9^a	52.7 ± 1.5^b	56.5 ± 7.7^a	32.3 ± 3.0^a	1.89 ± 0.03^a,b	71
UAS-I_h^RNAⁱ/+	919.0 ± 29.5^a	444.5 ± 15.9^b	474.9 ± 17.3^a	16.9 ± 1.1^a	58.7 ± 1.6^a	19.9 ± 4.0^b	26.5 ± 5.1^b	1.80 ± 0.03^b	70
R6-Gal4>UAS-I_h^RNAⁱ	1111.7 ± 27.7^a	545.9 ± 15.7^a	551.2 ± 14.9^a	27.2 ± 0.6^a	53.3 ± 2.9^a	6.8 ± 2.6^b	30.9 ± 7.2^a	2.21 ± 0.09^a	65	3	25
R6-Gal4/+	854.5 ± 35.9^b	409.7 ± 18.0^b	440.9 ± 20.8^b	15.6 ± 1.2^b	58.2 ± 2.3^a	19.0 ± 3.0^a	26.6 ± 4.4^a	2.06 ± 0.03^a	71
UAS-I_h^RNAⁱ/+	826.9 ± 28.1^b	400.6 ± 16.4^b	423.6 ± 15.5^b	14.1 ± 2.9^b	59.9 ± 1.4^a	32.9 ± 5.4^a	24.2 ± 2.5^a	1.81 ± 0.03^b	70
pdf-Gal4, UAS-dicer2>UAS-I_h^RNAⁱ	715.9 ± 37.8^b	330.6 ± 20.7^b	385.3 ± 21.6^b	14.3 ± 0.9^b	52.2 ± 1.9^a	27.5 ± 6.2^b	36.5 ± 6.2^a	1.75 ± 0.04^b	64	2	25
pdf-Gal4, UAS-dicer2/+	714.0 ± 29.3^b	320.8 ± 15.3^b	393.3 ± 18.3^b	14.0 ± 0.7^b	52.4 ± 1.6^a	70.0 ± 7.1^a	42.4 ± 6.3^a	1.73 ± 0.03^b	56
UAS-I_h^RNAⁱ/+	937.5 ± 28.1^a	414.3 ± 18.4^a	525.5 ± 15.2^a	18.7 ± 0.9^a	52.6 ± 1.7^a	39.0 ± 6.5^b	21.3 ± 4.6^b	1.89 ± 0.03^a	64

The following sleep parameters on the different genetic manipulations presented in the first column are shown: total sleep, daytime sleep, nighttime sleep, sleep bout duration, bout amount, latency to lights on, latency to lights off, and activity index (defined as the average activity counts in the active minutes). The last column shows the temperature at which each experiment was performed. Average ± SEM (Standard Error of the Mean) of N experiments using a final n number of individuals are displayed. Different letters indicate significant differences (p < 0.05) after non parametric Kruskal–Wallis statistical analysis with multiple comparisons (p adjustment method = BH). Sleep parameters where the experimental genotype showed statistically significant differences compared with genetic controls are displayed in bold.