Figure 4.

Delayed onset of P2RY1-dependent spontaneous crenations in ISCs. A, Intrinsic optical imaging performed before and after application of the P2RY1 antagonist, MRS2500 (1 μm). Detected crenations are outlined in colors based on time of occurrence as indicated by timeline below image. Imaging was performed near physiological temperature (32°C-34°C). B, Plot of crenation frequency and area before and after application of MRS2500. n = 6 E16-17 videos, 6 cochleae from 6 mice, n = 5 apical P0-2 videos, 5 cochleae from 5 mice, n = 6 basal P0-2 videos, 6 cochleae from 6 mice, n = 5 apical P6-8 videos, 5 cochleae from 5 mice, and n = 5 apical P10-12 videos, 5 cochleae from 5 mice. ****p < 5e-5; ***p < 5e-4; one-way ANOVA with Tukey post hoc. *p < 0.05, **p < 0.005.