Table 1. Preparation of the BSA standards.

Tube	BSA concentration (mg/ml)	H2O volume (µl)	BSA volume (µl)
A	2.000	0	300 from stock solution 2 mg/ml
B	1.500	125	375 from stock solution 2 mg/ml
C	1.000	325	325 from stock solution 2 mg/ml
D	0.750	175	175 from tube B
E	0.500	325	325 from tube C
F	0.250	325	325 from tube E
G	0.125	325	325 from tube F
H	0.025	400	100 from tube G
I	0	400	0