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. 2020 Feb 5;10(3):e3513. doi: 10.21769/BioProtoc.3513

Figure 9. His6PKD1-2 elution from Superdex 75 SEC.

Figure 9.

Comparison of the elution times with Figure 8 indicates that the larger left composite peak corresponds to higher order oligomers exceeding > 75 kDa and approximately 670 kDa. The largest peak, corresponding to dimers of His6PKD1-2, is used, and there is only the faintest hint of something approximating a monomer eluting later.