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. 2020 May 5;10(9):e3605. doi: 10.21769/BioProtoc.3605

Figure 3. Two different assays to quantify intestinal colonization by bacteria in Caenorhabditis elegans.

Figure 3.

A. Colony Forming Units (CFU) per worm growing in E. coli OP50 (white) and Salmonella Typhimurium MST1 (red) for two generations. Unpaired t-test, P < 0.0001. N = 90 worms per biological replica, each column contains three replicates. B. Percentage of L4 worms showing different phenotypes of intestinal colonization when grown on E. coli OP50-GFP and S. Typhimurium MST1-GFP. Full, partial and undetectable are defined in Procedure D. Two-way ANOVA, Holm-Sidak test, P < 0.05. N = 45 worms per biological replica.