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. 2021 Jan 28;16(1):e0245699. doi: 10.1371/journal.pone.0245699

Fig 2. Specificity of the PPV mPCR method.

Fig 2

Agarose gel electrophoresis (2%) of specific fragments amplified by mPCR from the proviral DNA and cDNA of Pk-15 cells, PRRS (NA), PRRS (EU), PCV2, PED/TGE/Rota-mixed (vaccine), E. coli, Salmonella enterica and JEV. Lane M, 100 bp-plus DNA ladder; lane NC, negative control; lane 1, Pk-15 cells; lane 2, PRRS (NA); lane 3, PRRS (EU); lane 4, PCV2; lane 5, PED/TGE/Rota-mixed (vaccine); lane 6, E. coli; lane 7, Salmonella enterica; lane 8, JEV; lane 9, PRV; lane 10, CSFV; lanes 11~17, pGEM-PPV1, pGEM-PPV2, pGEM-PPV3, pGEM-PPV4, pGEM-PPV5, pGEM-PPV6 and pGEM-PPV7; lane 18, mixed standard of all pGEM-PPV plasmids.