Table 2.

Solvents delivered for the two-dimensional UHPLC method.

Loading pump			Eluting pump
Time [min]	Flow [mL/min]	Mobile phase B1 [%]	Time [min]	Flow [mL/min]	Mobile phase B2 [%]
0.00	1.5	0	0.00	0.6	0
1.00	1.5	0	1.50	0.6	0
1.01	0.1	0	1.51	0.6	50
2.00	0.1	50	2.50	0.6	50
2.01	1.5	50	2.51	0.6	100
2.50	1.5	50	3.90	0.6	100
2.60	1.5	100	3.91	0.6	0
3.90	1.5	100	5	0.6	0
4.00	1.5	0
5.00	1.5	0