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. 2021 Jan 21;2021:8888913. doi: 10.1155/2021/8888913

Figure 2.

Figure 2

Dose- and time-dependent effects of CYR61 on the production of IL-6 and CCL20 in GO orbital fibroblasts. (a, b) GO orbital fibroblasts were treated with different doses (0, 2, 5, and 10 μg/ml) of CYR61 for 24 h. IL-6 mRNA (a) and CCL20 mRNA (b) expressions were examined by real-time PCR. (c) GO orbital fibroblasts were treated with 2 μg/ml CYR61 for 0, 24, 48, or 72 h. IL-6 mRNA expression was determined by real-time PCR. (d) GO orbital fibroblasts were treated with 5 μg/ml CYR61 for 0, 24, 48, or 72 h. CCL20 mRNA expression was determined by real-time PCR. (e, f) GO orbital fibroblasts were treated with 5 μg/ml CYR61 for 24 and 48 h. The protein levels of IL-6 (e) and CCL20 (f) in the culture supernatant were measured by ELISA. Data are presented as mean ± SD from at least three independent experiments. ∗p < 0.05, ∗∗p < 0.01.