Figure 1. Concurrent ablation of Satb1 and increased expression of Notch1 in mature T cells results in lethal adenopathy.

(A) Representative sizes of different organs in CD11c^CreSatb1^fl/flRosa26^N1-ICD mice, mice with CD11c^Cre-dependent Notch1 (N1-ICD) overexpression alone (CD11c^CreRosa26^N1-ICD), and mice with CD11c^Cre-induced ablation of Satb1 alone (CD11c^CreSatb1^fl/fl). LN, axillary lymph nodes. (B) Survival curve of CD11c^CreSatb1^fl/flRosa26^N1-ICD mice (n = 18), mice with CD11c^Cre-dependent Notch1 overexpression alone (CD11c^CreRosa26^N1-ICD) (n = 12), mice with CD11c^Cre-dependent Satb1 ablation alone (CD11c^CreSatb1^fl/fl) (n = 10), and mice without CD11c^Cre (n = 11). Log-rank (Mantel-Cox) test: ****P < 0.0001. (C) Weight of different organs in CD11c^CreSatb1^fl/flRosa26^N1-ICD mice (n = 3–5), CD11c^CreRosa26^N1-ICD mice (n = 5–7), CD11c^CreSatb1^fl/fl mice (n = 4–5), or mice without Notch1 overexpression or Satb1 ablation (CD11c^Cre negative) (n = 7). One-way ANOVA with Tukey’s multiple-comparison test: *P < 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001. (D) Representative hematoxylin and eosin staining of kidneys of CD11c^Cre-negative mouse (left) versus CD11c^CreSatb1^fl/flRosa26^N1-ICD mice (right).