Figure 2. Satb1 ablation transforms the Notch-dependent expansion of CD8⁺ T cells into a full-blown CD4⁺CD8⁺ T cell lymphoma.

(A) Notch-overexpressing (GFP⁺) cells in the peripheral blood of 10-week-old CD11c^CreSatb1^fl/flRosa26^N1-ICD mice are CD56^–TCRγδ^–CD3⁺ T cells. (B) An initial population of GFP⁺CD8⁺ T cells overexpressing Notch1 progressively transforms into CD4⁺CD8⁺ T cells at advanced stages of malignant progression, with quantitative representation of CD3⁺GFP⁺ T cells from peripheral blood. (C) Progressive accumulation of CD4⁺CD8⁺ T cells in the peripheral blood of CD11c^CreSatb1^fl/flRosa26^N1-ICD mice. (D) Progressive expansion of CD8⁺ T cells, but not CD4⁺CD8⁺ malignant lymphocytes, in Notch-overexpressing mice without Satb1 ablation. (E) Quantitative representation of progressive accumulation of CD4⁺CD8⁺, CD4⁺, and CD8⁺ T cells in CD11c^CreSatb1^fl/flRosa26^N1-ICD mice (n = 4–12), CD11c^CreRosa26^N1-ICD (n = 4–6), and CD11c^Cre-negative mice (n = 6–9). Two-tailed Student’s t test (B and E): **P ≤ 0.01.