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. 2021 Feb 1;131(3):e140315. doi: 10.1172/JCI140315

Figure 4. Macrophage-mediated permeability is dependent on RAC1 and p-PYK2.

Figure 4

(A) Protein levels of RAC1 and p-PYK2 in HUVECs from different cocultures examined by Western blot (n = 3). (B) Immunofluorescence analysis of RAC1 in HUVECs and HUVECs cultured with different subtypes of macrophages. Scale bar: 20 μm. (C) RAC1 and p-PYK2 protein expression in HUVECs from M1 macrophage–cocultured system treated with VLA4 inhibitor CDP323 (n = 3). (D) RAC1 and p-PYK2 expression in HUVECs from M1 macrophage–cocultured system treated with VCAM1 inhibitor K-7174 (n = 3). (E) Flow cytometric analysis of ROS levels in HUVECs from M1 macrophage–cocultured system treated with RAC1 inhibitor NSC23766 (n = 3). (F and G) p-PYK2 expression in HUVECs from M1 macrophage–cocultured system treated with NAC (F) or and M2 macrophage–cocultured system treated with H2O2 (G) (n = 3). Results represent 3 independent experiments. Results are shown as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, Student’s t test (C–G) and 1-way ANOVA (A).