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. 2021 Feb 1;131(3):e139333. doi: 10.1172/JCI139333

Figure 4. Cytoplasmic release of mtDNA is responsible for ATM inhibition–induced cGAS/STING activation.

Figure 4

(A) WB analysis of protein levels of p-TBK1 and STING in vector control and Atm-KO B16F10 cells exposed to 100 ng/mL EthBr for 20 days to deplete mtDNA. (B) qPCR analysis of IFN response gene expression in vector control and Atm-KO B16F10 cells that had been treated with 100 ng/mL EthBr for 20 days. Data represent the mean ± SEM. *P < 0.05, by 2-way ANOVA. n = 3. (C) WB analysis of p-TBK1 levels in B16F10 cells that had been treated with sham or 100 ng/mL EthBr for 20 days and then exposed to 1 μM AZD1390 for 48 hours. GAPDH was used as a protein loading control. (D) Vector control and Atm-KO B16F10 cells that had been treated with 100 ng/mL EthBr for 20 days were costained with anti-dsDNA (green), anti-Hsp60 (red), and DAPI. Scale bars: 10 μm. Original magnification, ×12 (insets).