PBMCs from P1 taken at various time points were stained for surface expression of CD4, CD8, CD20, CCR7, CD45RA, CD10, and CD27, fixed, permeabilized, and intracellularly stained to detect DOCK8. Percentages of cells expressing DOCK8 in (A) total lymphocytes; (B) total, transitional, naive, and memory B cells; (C) total, naive, and memory CD4+ T cells; and (D) total, naive, Tcm, Tem, and Temra CD8+ T cells were determined by flow cytometry.