Figure 3.

MiR‐488 targeted PFKFB3 in colorectal cancer (CRC) cell lines. A, The miR‐488 level and mRNA level of PFKFB3 were simultaneously abnormally expressed among 450 CRC samples, according to ENCORI software. B, The binding sites between PFKFB3 and miR‐488 were predicted by targetscan website. C, MiR‐488 expression was determined among NCM460, SW260, HT‐29, Lovo, and HCT116 cell lines. *: P < .05 when compared with NCM460 cell line. D, MiR‐488 level was heightened in CRC cell lines after transfection of miR‐488 mimic. *: P < .05 when compared with miR‐NC group. E, The luciferase activity of HCT116 and Lovo cells was weakened when PFKFB3 Wt and miR‐488 mimic were co‐transfected. *: P < .05 when compared with PFKFB3 Wt + miR‐NC group. F, The mRNA level of PFKFB3 was reduced by miR‐488 mimic. *: P < .05 when compared with miR‐NC group. G, The mRNA level of PFKFB3 was up‐regulated by transfection of pcDNA3.1‐PFKFB3. *: P < .05 when compared with pcDNA3.1 group. H, MiR‐488 level was determined when pcDNA3.1‐PFKFB3 was transfected. *: P < .05 when compared with pcDNA3.1 group