TABLE 2.
The results of the LAMP assay compared to those of the RUT and PCR and sequencing analysis for the diagnosis of H pylori and the detection of the A2143G and T2182C point mutations in 23S rRNA
| Development cohort | RUT | PCR | ||||
|---|---|---|---|---|---|---|
| LAMP for H pylori a | Negative | Positive | Statistics b | Negative | Positive | Statistics |
| Negative | 343 | 5 | P = .017 | 348 | 0 | P = 1.0 |
| Positive | 17 | 229 | κ = 0.923 | 0 | 246 | κ = 1.0 |
| LAMP for 2143G or 2182C | Sequencing A2143G | Sequencing T2182C | |||||
|---|---|---|---|---|---|---|---|
| 2143A | 2143G | Statistics | 2182T | 2182C | Statistics | ||
| 2143G | Negative | 182 | 0 | P = 1.0 | |||
| Positive | 0 | 64 | κ = 1.0 | ||||
| 2182C | Negative | 30 | 0 | P = 1.0 | |||
| Positive | 0 | 216 | κ = 1.0 | ||||
a
DNA used in LAMP analysis was extracted in a laboratory setting with a QIAamp® DNA Mini Kit (QIAGEN, #51306). Twenty‐eight patients with an extracted DNA concentration of <50 ng/µL were excluded from the PCR and LAMP analyses.
b
P from McNemar test and κ from unweighted pairwise Cohen's Kappa.