TABLE 3.
A comparison of the direct LAMP assay with the RUT and PCR and sequencing analysis for the diagnosis of H pylori and the detection of the A2143G and T2182C point mutations in 23S rRNA
| Direct LAMP cohort | RUT | PCR | ||||
|---|---|---|---|---|---|---|
| Direct LAMP analysis a | Negative | Positive | Statistics b | Negative | Positive | Statistics |
| Negative | 49 | 2 | P = 1.0 | 50 | 1 | P = 1.0 |
| Positive | 1 | 41 | κ = 0.935 | 0 | 42 | κ = 0.978 |
| LAMP for 2143G or 2182C | Sequencing A2143G | Sequencing T2182C | |||||
|---|---|---|---|---|---|---|---|
| 2143A | 2143G | Statistics | 2182T | 2182C | Statistics | ||
| 2143G | Negative | 29 | 1 | P = 1.0 | |||
| Positive | 0 | 12 | κ = 0.943 | ||||
| 2182C | Negative | 8 | 1 | P = 1.0 | |||
| Positive | 0 | 33 | κ = 0.926 | ||||
a
Gastric mucosal DNA was directly extracted from a biopsy sample at the bedside with the Mmaxpress® DNA kit HS2 included in the Isopollo® H pylori & ClaR kit (Mmonitor Inc).
b
P from McNemar test and κ from unweighted pairwise Cohen's Kappa.