Figure 2.
Increased PMN - to - Candida ratio leads to a non-significant decrease of the fungal burden after 180 min. (A) Colony forming units were determined on YPD plates from infected blood samples at various time points for 3 h. All values correspond to the means of three independent experiments with pooled whole blood from 10 BALB/c mice infected ex vivo with 1 × 105/ml of C. albicans::gfp. Each dot represents the mean of an independent experiment ± standard deviation (n = 3). (B) Colony forming units were counted in murine whole blood as described for (A) with the following modifications: the infection dose was 1 × 106/ml and murine whole blood was supplemented with 4.5 × 106/ml neutrophils that were previously isolated from bone marrow of BALB/c mice. Although differences between control and neutrophil supplemented blood were observed at later time points, these were not statistically significant (p>0.05, Wilcoxon matched-pairs signed rank test).