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. 2021 Jan 15;11:565869. doi: 10.3389/fimmu.2020.565869

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Copyright © 2021 Machata, Sreekantapuram, Hünniger, Kurzai, Dunker, Schubert, Krüger, Schulze-Richter, Speth, Rambach and Jacobsen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interaction of isolated murine platelets with C. albicans. (A) Binding of isolated platelets to C. albicans cells in the presence of heat-inactivated serum (inactive serum) or non-treated serum (active serum). The percentage of CD41-positive C. albicans cells was determined after 1 h by flow cytometry. Active serum led to a significantly higher interaction with platelets compared inactive serum and a control without serum (asterisks indicate significant differences (p<0.05) determined by 1-Way ANOVA and Holm-Sidak’s multiple comparison test for pairwise comparisons of all groups). (B) Binding of isolated platelets to C. albicans cells in the presence of active serum from naïve mice, serum of mice collected after infection with C. albicans (reconvalescent) and serum from naïve C3-deficient mice analyzed after 1 h and 2 h. Reconvalescent serum led to significantly lower binding (significance determined by 1-Way ANOVA and Holm-Sidak’s multiple comparison test for pairwise comparisons of all groups within one time point and indicated by asterisks).