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. 2021 Jan 15;12:614587. doi: 10.3389/fnagi.2020.614587

FIGURE 4.

FIGURE 4

Short term localization of tau K18 PFFs in the eye post IVT injection. (A) Histological examination of sagittal eye sections with the PT76 antibody demonstrates the localization of tau K18 PFFs (arrows). Upper row: in the condition where tau K18 PFFs were injected, PT76 staining can be observed in the vitreous body while no staining in the retina is apparent at 2, 4, 8, 24, and 48 h post IVT injection. In the PBS injected condition, no PT76 staining is observed in the vitreous body or the retina at 48 h post IVT injection. Lower row: sagittal sections of the retina, with the retinal layers highlighted, show the lack of any PT76 signal in the tau K18 PFFs conditions. (B–E) Immunostaining of infiltrating immune cells (CD45) on sagittal eye sections shows co-localization with PT76 at 24 and 48 h post K18 PFF IVT injection. (F,G) At 48 h post IVT injection of the PBS control, PT76 staining is absent in the vitreous and infiltration of CD45+ inflammatory cells is limited. Scalebar: 100 μm, n = 4 per time point. L, lens; R, retina; GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; ONL, outer nuclear layer.