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. 2020 Oct 17;11(3):683–696. doi: 10.1016/j.jcmgh.2020.10.007

Figure 6.

Figure 6

Fg promotes cell-to-cell disaggregation in vitro. (A, B) MS1 cells were stimulated with or without Fg (5 mg/mL) for (A) indicated times or (B) 24 hours. (A) Cells were photographed by time lapse. Scale bar = 300 μm. (B) Cell death was analyzed by PI/Hoechst staining: cell death (%) = (PI positive cells / Hoechst positive cells) ×100%. Scale bar = 50 μm. (C) Fg decreased distribution of microfilaments at the cell junction. MS1 cells were stimulated with or without Fg for 12 hours and stained with phalloidin. Scale bar = 10 μm. (D, E) Fg induced microfilament depolymerization in MS1 cells. MS1 cells were stimulated with Fg (5 mg/mL) for indicated times, (D) G-actin and F-actin were detected by fractionation and immunoblotting, and (E) densitometric analysis of actin level by the ImageJ program. In B and E, data are presented as mean ± SEM. ∗∗P < .01; ∗∗∗P < .001; ns, P >.05 (2-tailed unpaired Student’s t test).