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phoP is required for Pve polymyxin resistance. Wild type and phoP mutant Pve cultures were grown aerobically in LB medium to mid-log phase, diluted to 105 cells ml–1 and split in two. 0.2 μg/ml of polymyxin B was added to one of the split cultures and incubation continued for 4 more hours. Surviving cell numbers were determined by plating. Mean values of three biological replicates and 95% confidence intervals are shown.
