Table 1.

Reporters used with recombinant vesicular stomatitis virus (VSV) lacking the envelope glycoprotein (rVSVΔG).

Gene reporters for pseudotyping	Methodology of detection	Advantages	Disadvantages
Pseudotyped ΔG-GFP (G*ΔG-GFP) rVSV	Fluorescence	- Live-cell imaging - Cytotoxicity evaluation - Cells usable for RNA/protein work - Flourescence microscope
Pseudotyped ΔG-DsRed (G*ΔG-DsRed) rVSV	Fluorescence	- Live-cell imaging - Cytotoxicity evaluation - Cells usable for RNA/protein work - Flourescence microscope
Pseudotyped ΔG-luciferase (G*ΔG-luciferase) rVSV	Luminescence assay for alkaline phosphatase (AP) activity	- Low cost for kits and reagents - Plate reader	- Background for pseudoviral infection
Pseudotyped ΔG-SEAP (G*ΔG-SEAP) rVSV	Luminescence/ Colorimetric assay for alkaline phosphatase (AP) activity	- Low cost for kits and reagents - Plate reader	- Background for pseudoviral infection - Internal alkaline phosphatase (AP) activity