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Development of screening system on a 96-well microplate. (A) Scheme of the random domain screening method. (B) EGFP released from SPN1S-induced lysis in host E. coli cells. Cells without induction IPTG for EGFP expression and arabinose for SPN1S lysRz expression) were used as a control. (C) Clones for positive and negative controls were cultured with 0.2% arabinose overnight on the lawn of autoclaved S. aureus RN4220.
