Fig. 7.

Occludin expression in RPE/choroid organ culture after treatment with TLR agonists for 24 h. Poly I:C but none of the other agonists significantly influenced the intensity of the occludin signal (a). Immunofluorescent occludin pictures show a redistribution of occludin into the cell body (b control, c Pam 100 ng/ml, e Poly I:C 100 μg/ml, f LPS 10 μg/ml), which is highly significant in semi-quantitative analysis for Poly I:C and LPS (d). Representative examples are given in b control, c Pam, e Poly I:C, and f LPS. Scale bar indicates 20 μm (n = 15), Significance evaluated with the Kruskal–Wallis and Mann–Whitney U tests, ⁺p < 0.05, ⁺⁺⁺p < 0.001, compared with control. Pam 100, Pam 100 ng/ml; Poly 100, Poly I:C 100 μg/ml; LPS 10, LPS 10 μg/ml