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. 2020 Dec 23;45:101150. doi: 10.1016/j.molmet.2020.101150

Figure 2.

Figure 2

Etomoxir and DGAT1 inhibition have similar effects on FA 11:0;Y-TG formation. Hepatocytes (75,000 per well) were pre-incubated with either vehicle (con) or 3 μM of DGAT1 inhibitor (D1i), 15 μM of DGAT2 inhibitor (D2i), 50 μM of etomoxir (Eto) or a combination of both inhibitors with etomoxir (D1i + Eto or D2i + Eto) for 1 h. Cells were then incubated for 1 h with a combination of 50 μM of FA 11:0;Y and 50 μM of FA 19:1;Y. The cells were washed, and lipids were extracted and analysed by click reaction with azidocoumarin followed by (A, C) TLC separation and fluorescent imaging. (B, D) Normalised quantification of fluorescent intensities from FA 11:0;Y-TG (filled bars) and FA 19:1;Y-TG (grey hatched bars) upon either etomoxir (orange), D1i (red), D2i (blue) and a combination of both inhibitors (orange/red or orange/blue) in comparison to the negative controls (black: FA 11:0;Y, grey: FA 19:1;Y). The data represent mean ± SD for n = 3 biological replicates. ∗p ≤ 0.0332, ∗∗p ≤ 0.0021, ∗∗∗∗p ≤ 0.0001. ns = not significant.