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. 2020 Dec 23;45:101150. doi: 10.1016/j.molmet.2020.101150

Figure 3.

Figure 3

Analyses of alkyne-labelled lipid species from wild-type (WT) or DGAT1−/−hepatocytes, incubated with alkyne-palmitate and decanoic acid. Hepatocytes (75,000 per well) were co-incubated with 50 μM of FA 17:0;Y and 50 μM of decanoic acid (FA 10:0) for 1 h. Lipids were extracted, and alkyne-labelled species were identified and quantified by multiplexed click-MS analysis as described in the Materials and Methods section. Absolute amounts of the lipid species within the lipid classes of (A) TG;Y, (B) DG;Y and (C) PC;Y are shown in pmol. The data represent mean values for n = 3 biological replicates.