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. 2020 Dec 23;45:101150. doi: 10.1016/j.molmet.2020.101150

Figure 4.

Figure 4

Analyses of alkyne-labelled TG species from wildtype (WT) or DGAT1−/−hepatocytes, treated with inhibitor combinations and incubated with alkyne-palmitate and decanoic acid. Hepatocytes (75,000 per well) were pre-incubated with either vehicle (con) or 50 μM of etomoxir (Eto), 3 μM of DGAT1-inhibitor (D1i), 15 μM of DGAT2-inhibitor (D2i) or combinations of etomoxir with each DGAT-inhibitor (Eto + D1i, Eto + D2i). Cells were then co-incubated with 50 μM of FA 17:0;Y and 50 μM of decanoic acid (FA 10:0) for 1 h. Lipids were extracted, and alkyne-labelled species were identified and quantified by multiplexed click-MS analysis as described in the Materials and Methods sections. (A) MCFA-TG;Y (∑C41–C47) and (B) LCFA-TG;Y (∑C49-57). The data represent mean ± SD for n = 3 biological replicates. ∗p ≤ 0.0332, ∗∗p ≤ 0.0021 and ∗∗∗∗p ≤ 0.0001. ns, not significant.