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. 2020 Dec 23;45:101150. doi: 10.1016/j.molmet.2020.101150

Figure 6.

Figure 6

MCFA-TG and LCFA-TG formation upon incubation with alkyne-palmitate and FA 10:0 or 12:0 in the presence of various inhibitors. Hepatocytes (75,000 per well) were pre-incubated with either vehicle (con) or etomoxir (Eto), teglicar (Tegli) or DGAT inhibitors (D1i, D2i). Numbers indicate inhibitor concentrations in μM. Cells were co-incubated with (A,B) 50 μM of FA 17:0;Y and 50 μM of decanoic acid (FA 10:0) or (C,D) 50 μM of FA 17:0;Y and 50 μM of FA 12:0 for 1 h in the presence of the respective inhibitors. Lipids were extracted, and alkyne-labelled species were identified and quantified by multiplexed click-MS analysis as described in the Materials and Methods section. (A,C) MCFA-TG;Y and (B,D) LCFA-TG;Y were quantified and are expressed as % of control. The data represent mean ± SD for n = 3 biological replicates. ∗p ≤ 0.05, ∗∗p ≤ 0.01 and ∗∗∗p ≤ 0.001. ns, not significant.