Skip to main content
. 2021 Jan 15;8:556004. doi: 10.3389/fcell.2020.556004

FIGURE 4.

FIGURE 4

miR-202-3p suppresses HepG2 cell viability, migration and invasion in vitro by the KDM3A/HOXA1/MEIS3 pathway. (A) The mRNA level of KDM3A, HOXA1, and MESI3 in KDM3A knockdown and/or HOXA1 overexpression HepG2 cells by qRT-PCR analysis. * (compared with scramble siRNA + oe-NC) and # (compared with KDM3A siRNA + oe-NC) indicate p < 0.05 by Tukey’s test-corrected one-way ANOVA. (B–D) The miR-202-3p expression, Western blots, and quantification of KDM3A, HOXA1, and MESI3 in HepG2 cells treated with miR-202-3p mimic alone or with HOXA1 recombinant lentiviral expression vectors by qRT-PCR and Western blot analysis. (E) HepG2 cell viability was evaluated by MTT assay at indicated time points. (F,G) Representative view (×200) of HepG2 cells migrating from upper transwell chambers without Matrigel into lower ones and statistics of migrating cells; representative view (×200) of HepG2 cells invading from Matrigel-coated upper transwell chambers into lower ones and statistics of invading cells. * (compared with mimic-NC + oe-NC) and # (compared with miR-202-3p mimic + oe-NC) indicate p < 0.05 by Tukey’s test-corrected one-way ANOVA for (B–D) and (F,G) and by Bonferroni-corrected repeated measures ANOVA for (E).