TABLE 2.
Treatment | Fresh root weight (g plant–1) | Fresh shoot weight (g plant–1) | Leaf number (leaf plant–1) | Plant height (cm plant–1) | Stem diameter (mm plant–1) | Leaf area (cm2 plant–1) | |||||||
Stress conditions | Mycorrhizal inoculation | 4 weeks | 8 weeks | 4 weeks | 8 weeks | 4 weeks | 8 weeks | 4 weeks | 8 weeks | 4 weeks | 8 weeks | 4 weeks | 8 weeks |
Non-stress | AMF- | 1.17 ± 0.05ab | 1.68 ± 0.56b | 2.43 ± 0.42b | 4.4 ± 0.7b | 10.0 ± 1.5c | 15.2 ± 1.5bc | 11.3 ± 6.0a | 24.6 ± 6.6ab | 2.0 ± 0.3b | 2.93 ± 0.2b | 8.73 ± 0.7b | 11.93 ± 2.8bc |
AMF + | 1.68 ± 0.4a | 2.95 ± 0.72a | 4.02 ± 0.4a | 9.9 ± 2.6a | 18.9 ± 2.9a | 31.5 ± 10.1a | 13.7 ± 6.0a | 25.5 ± 6.5a | 3.4 ± 0.7a | 4.31 ± 0.8a | 15.85 ± 2.6a | 23.81 ± 3.7a | |
100 mM NaCl | AMF- | 0.53 ± 0.3bcd | 0.15 ± 0.05c | 0.99 ± 0.33cd | 1.4 ± 0.1c | 9.4 ± 0.4c | 11.1 ± 1.5c | 6.9 ± 2.2a | 10.9 ± 3.0c | 1.9 ± 0.1b | 2.13 ± 0.1b | 5.24 ± 0.8cd | 7.63 ± 1.0c |
AMF + | 0.78 ± 0.3bc | 0.41 ± 0.06c | 1.82 ± 0.6bc | 2.7 ± 0.3b | 15.8 ± 3.2ab | 21.8 ± 3.7b | 7.9 ± 2.1a | 13.3 ± 2.2bc | 2.4 ± 0.1ab | 2.45 ± 0.1b | 8.37 ± 0.6b | 14.38 ± 3.7b | |
200 mM NaCl | AMF- | 0.03 ± 0.00d | 0.13 ± 0.02c | 0.44 ± 0.1d | 0.9 ± 0.4c | 9.4 ± 0.5c | 9.0 ± 1.7c | 3.8 ± 0.5a | 5.0 ± 0.6c | 1.4 ± 0.2b | 2.06 ± 0.4b | 3.28 ± 0.6d | 7.28 ± 1.5c |
AMF + | 0.11 ± 0.02cd | 0.19 ± 0.04c | 0.66 ± 0.5cd | 1.6 ± 0.6bc | 12.8 ± 1.1bc | 11.7 ± 0.3c | 5.6 ± 0.7a | 10.1 ± 2.1c | 1.8 ± 0.3b | 2.36 ± 0.4b | 6.60 ± 0.5bc | 9.15 ± 1.8bc | |
Source of variation (ns, not significant, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001) | |||||||||||||
Mycorrhizal inoculation (M) | * | * | *** | *** | *** | *** | ns | ns | ** | ** | *** | *** | |
Salt stress (S) | *** | *** | *** | *** | ** | ** | * | *** | *** | *** | *** | *** | |
M × S | ns | * | ns | ** | * | ns | ns | ns | ns | Ns | * | * |
The means ± standard deviation are presented (n = 5 for mycorrhizal colonization, n = 3 for other parameters). Different letters in each column indicates significant difference according to the Tukey test (P < 0.05) among treatments 4 and 8 weeks after inoculation. AMF, arbuscular mycorrhizal fungi; ns, non-significant difference.