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. 2021 Jan 28;11:2593. doi: 10.1038/s41598-021-82219-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of orogastric gavage of neonatal SP-A^−/− mice with purified SP-A. (A) Western analysis to detect the presence of SP-A was performed on 1 week and 4 week old WT and SP-A^−/− mice 2 h after gavage with 50 µg of purified SP-A. (B) 1 week old SP-A^−/− mice that were untreated, gavaged with PBS or with 5 µg of purified SP-A in PBS on day 4 and day 6 of life. Shown is Western analysis of TLR4 and β-actin protein in the ileum of 3 mice from each treatment regimen. Figure created using SigmaPlot, ver 14, www.systatsoftware.com.