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. 2021 Jan 28;11:2593. doi: 10.1038/s41598-021-82219-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of SP-A exposure on expression of TLR4 and proinflammatory cytokine mRNA in human intestinal epithelial (FHs74int) cells in culture. FHs74int cells were cultured in the absence of presence of SP-A (25 µg/ml) for 24 h. Shown are the results of real-time quantitative RT-PCR analysis of RNA isolated from the cells. All results were normalized to levels determined in untreated cells (set as 1). Shown are the results of 2 determinations, n ≥ 5. * indicates P < 0.05. Figure created using SigmaPlot, ver 14, www.systatsoftware.com.