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. 2021 Jan 28;11:2593. doi: 10.1038/s41598-021-82219-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of SP-A exposure on TLR4 mRNA and protein levels in 293/hTLR4-HA cells. (A) Real-time quantitative RT-PCR analysis of TLR4 mRNA in HEK293 cells (that do not express TLR4) and in 293/hTLR4-HA cells that express HA-tagged TLR4 via an expression cassette, incubated in the absence or presence of SP-A (25 µg/ml) for 24 h. Shown are the levels of TLR4 mRNA relative to untreated 293/hTLR4-HA cells (n = 6 from 2 assays). (B) Western analysis of HA-tagged TLR4protein expressed from 293/hTLR4-HA cells incubated in the absence or presence of SP-A (25 µg/ml) for 24 h. Shown are the results of western analysis using antibodies specific for HA and β-actin following PAGE of cellular protein. Figure created using SigmaPlot, ver 14, www.systatsoftware.com.