Skip to main content
. 2021 Jan 28;11:2593. doi: 10.1038/s41598-021-82219-y

Table 1.

Sequence of primers used in real-time quantitative RRT-PCR analysis.

Target Forward primer Reverse primer Amplicon
Human
TLR4 5′-TTTGGACAGTTTCCCACATTGA 5′-AAGCATTCCCACCTTTGTTGG 75 bp
IL-1β 5′-ACAGATGAAGTGCTCCTTCCA 5′-GTCGGAGATTCGTAGCTGGAT 72 bp
IL-6 5′-AATTCGGTACATCCTCGACGG 5′-TTGGAAGGTTCAGGTTGTTTTCT 112 bp
IL-8 5′-ATGACTTCCAAGCTGGCCGTGGCT 5′-TCTCAGCCCTCTTCAAAAACTTCTC 291 bp
Mouse
TLR4 5′-TTTATTCAGAGCCGTTGGTG 5′-CAGAGGATTGTCCTCCCATT 185 bp
IL-1β 5′-GCCACCTTTTGACAGTGATGAG 5′-AAGGTCCACGGGAAAGACAC 218 bp
IL-6 5′-TAGTCCTTCCTACCCCAATTTCC 5′-TTGGTCCTTAGCCACTCCTTC 75 bp
CXCL1 5′-CTGCACCCAAACCGAAGTC 5′-AGCTTCAGGGTCAAGGCAAG 66 bp
TNF-α 5′-CAGCCTCTTCTCATTCCTGC 5′-GGTCTGGGCCATAGAACTGA 132 bp
Common
18S 5′-GTAACCCGTTGAACCCCATT 5′-CCATCCAATCGGTAGTAGCG 150 bp