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. 2021 Jan 28;11:2546. doi: 10.1038/s41598-021-81858-5

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© Crown 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of (A) AG2-1 and (B) AG8 biomass in A. thaliana Col-0 roots and shoots using qPCR. Col-0 plants were inoculated by planting the roots directly into infected vermiculite. After DNA extraction, pathogen DNA was amplified using R. solani ITS primer sequences and Arabidopsis DNA amplified using beta-Tublulin primer sequences. The Y-axis represents relative abundance of R. solani DNA (picograms) versus A. thaliana DNA (nanograms) determined using a standard curve derived from cultured R. solani tissue and A. thaliana genomic DNA. Bars with differing letters represent a significant difference (p < 0.05) using a one-way ANOVA and Tukey’s HSD test. Six biological replicates of forty plants were used per treatment and timepoint. Error bars represent standard error.