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. 2021 Jan 28;11:2546. doi: 10.1038/s41598-021-81858-5

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© Crown 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Loss of JA, ET and PEN2 mediated defense pathways compromise resistance to AG8. (A) Infection of Col-0, pen1, pen2, pen3, single double and triple mutants with AG8. (B) Infection of Col-0, coi1 ein2 pen2, eds1 pen3 and rar1 sgt1b pen3 mutants with AG8. (C) Infection of Col-0, coi1 ein2 pen2, coi1 ers1 pen2 and coi1 ers2 pen2 with AG8. Three replicate infections of agar grown plants were performed by transferring 4 plants per biological replicate into mock or R. solani infected pots to produce the data in this figure. Plants were scored for survival at 14 days post infection at which point surviving plants had started to flower. An additional independent experimental repeat of this experiment was also performed which confirmed the result (Supplementary Fig. S5). Letters represents significance (adj. p < 0.05) using Fisher’s Exact Test. Error bars represent standard error.