Fig. 2. FOXO3 is necessary for ROS-induced IFN-I response.

a WB for FOXO3 in NSPCs expressing non-targeted guide RNA (sg-NT) or Foxo3 targeted guide RNA (sg-Foxo3). b WB for STAT1 and TBK1 phosphorylation following 48 h treatment. NT: mock-treated control, PQ: 5 μM of paraquat. c IFNβ secretion in the media following 48 h treatment. Mean ± s.e.m. of three independent experiments. d qRT-PCR results for ISGs following 4 days of PQ treatment. Mean ± s.e.m. of three independent experiments. Representative IF (e) and WB (f) results for FOXO3 on either adenovirus for control or FOXO3^TA-infected NSPCs. Scale bar = 10 µm. g IFNβ secretion in the media following 72 h of control or FOXO3^TA adenovirus infection. Mean ± s.e.m. of three independent experiments. h qRT-PCR results for ISGs at 4 days after the infection of adenovirus. Mean ± s.e.m. of three independent experiments. Statistical significance was determined by one-way ANOVA for (c, d, and h), and by two-sided unpaired t-test for g Experiments for a, b, e, and f were repeated three times independently with similar results and representative images/blots are shown.