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. 2021 Jan 28;11:2502. doi: 10.1038/s41598-021-82125-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Verification and binding analysis of rNIE specific recombinant monoclonal antibody proteins. (a) Western blot analysis of recombinant monoclonal antibody proteins. (b) Antigen–antibody Western blot analysis. (c) Immunoassay analysis of recombinant monoclonal antibody proteins. (d) Antibody titration ELISA; Ab23, Ab14, Ab6, Ab5 are antibody clones against rNIE, Ab5B-XP and Ab4-R1 are antibody clones against BmSXP and BmR1 antigens respectively (e) Cross-reactivity ELISA. All the binding verifications were detected using Strep Tactin HRP conjugated antibody except for cross-reactivity ELISA was detected using anti-M13 HRP conjugated antibody.