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. 2021 Jan 28;12:647. doi: 10.1038/s41467-021-20904-2

Fig. 1. NUPR1 acts as a repressor of ferroptosis.

Fig. 1

a A NanoString technology-based screening of differentially expressed tumor-associated genes in PANC1 and BxPC3 cells following treatment with erastin (10 µM) for 24 h. b Top 5 upregulated genes. c, d Nupr1+/+ and Nupr1−/− mPDAC cells were treated with erastin or RSL3 in the absence or presence of ferrostatin-1 (1 µM), liproxstatin-1 (1 µM), Z-VAD-FMK (10 µM), or necrosulfonamide (1 μM) for 24 h, and then cell viability (c) and lipid ROS (d) was measured (n = 3 well/group, two-way ANOVA with Tukey’s multiple comparisons test on all pairwise combinations). e qPCR analysis of NUPR1 expression in indicated mPDAC cells (n = 3 well/group, one-way ANOVA with Tukey’s multiple comparisons test on all pairwise combinations). f Cell viability of indicated mPDAC cells following treatment with erastin or RSL3 for 24 h (n = 3 well/group, two-way ANOVA with Tukey’s multiple comparisons test on all pairwise combinations). Data in d–f are presented as mean ± SD. The results in c–f are representative of those from 2 to 3 independent experiments with three technical replicates each. The results in a are representative of those from one independent experiment with three technical replicates each.

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