Fig. 2. NUPR1 inhibits iron-dependent oxidative damage in ferroptosis.

a Fe²⁺ levels in indicated mPDAC cells following treatment with erastin or RSL3 for 24 h (n = 3 well/group, two-way ANOVA with Tukey’s multiple comparisons test on all pairwise combinations). b–e Indicated mPDAC cells were treated with erastin (10 µM) or RSL3 (1 µM) in the absence or presence of DFO (100 µM) or NAC (1 mM) for 24 h, and then intracellular MDA (b), intracellular 8-OHdG (c), extracellular HMGB1 (d), and cell viability (e) were assayed (n = 3 well/group, two-way ANOVA with Tukey’s multiple comparisons test on all pairwise combinations). f qPCR analysis of Steap3 mRNA in indicated mPDAC cells (n = 3 well/group, two-way ANOVA with Tukey’s multiple comparisons test on all pairwise combinations). g, h Indicated mPDAC cells were treated with erastin (10 µM) or RSL3 (1 µM) or vehicle (0.01% dimethyl sulfoxide) for 24 h, and then extracellular HMGB1 (g) and cell viability (h) were determined (n = 3 well/group, two-way ANOVA with Tukey’s multiple comparisons test on all pairwise combinations). Data in a–h are presented as mean ± SD. The results in a–h are representative of those from 2 to 3 independent experiments with three technical replicates each.