Table 2.
Table is showing precursor ion, the transition of the parent ion, retention time (RT), calibration range, R2, collision energy, LOD and LOQ for the isoprostanes analyzed in both positive and negative mode of ion polarity
| S. no. | Name | Precursor Ion [M + H]+/ [M-H]− | Transition | RT (min) | Calibration range (μg/ml) | R2 | CE (V) | LOD (μg/ml) | LOQ (μg/ml) |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 8-iso PGA1 | 335.5 | 335.51 → 112.98 | 9.64 | 0.01–50.00 | 0.9742 | 28 | 0.256 | 1.281 |
| 2 | Prostaglandin F2α | 353.4 | 353.48 → 193.26 | 11.92 | 0.01–50.00 | 0.9979 | 30 | 0.007 | 0.039 |
| 3 | 11-epi-Prostaglandin F2α | 355.5 | 355.50 → 314.97 | 26.13 | 0.01–50.00 | 0.9113 | 30 | 0.015 | 0.078 |
| 4 | Prostaglandin F1α | 357.5 | 357.51 → 105.03 | 23.52 | 0.01–50.00 | 0.9997 | 30 | 0.072 | 0.360 |
| 5 | PGF2α –d 4 | 357.5 | 357.52 → 112.98 | 28.41 | 0.01–50.00 | 0.9065 | 30 | 0.700 | 3.502 |
| 6 | 8-iso-16-cyclohexyl-tetranor-PGE2 | 377.5 | 377.50 → 190.94 | 28.10 | 0.01–50.00 | 0.9972 | 32 | 0.00095 | 0.0047 |
PGA1, Prostaglandin A1, PGF2α –d 4 Prostaglandin F2α-d4(Dinoprost-d4), PGE2 Prostaglandin E2