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. 2021 Jan 23;2(1):100301. doi: 10.1016/j.xpro.2021.100301

Figure 3.

Figure 3

Morphology of cortical microtubules visualized by live imaging and immunostaining

(A–C) Live imaging of CMTs in Arabidopsis sepal primordium after staining with FM4-64. (A) 3D reconstruction of CMT array at the sepal surface (periclinal walls). (B) FM4-64 signals at the surface. (C) Tilted image of the area framed in (A) representing the CMTs in anticlinal walls (arrowheads).

(D and E) Immunostaining of CMTs in a cross-section of tomato leaf primordium. (D) Double labeling of CMTs (green color) and nucleus (red color) in each cell by tubulin antibodies and DAPI, respectively. (E) The organization of CMTs in (D). Scale bars: 20 μm.