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MTO maintenance media
| Reagent | Final concentration | Stock concentration | Volume (mL) |
|---|---|---|---|
| Advanced DMEM/F12 | – | – | 95.95 |
| GlutaMAX supplement | 1% | 100% | 1 |
| HEPES | 1% | 100% | 1 |
| B27 supplement minus vitamin A (add before using) | 2% | 100% | 2 |
| Murine EGF (add before using) | 50 ng/mL | 100 μg/mL | 0.05 |
