FIGURE 6.

EREG regulates HSC activation through MRTF-A. (A) LX-2 cells were treated with EREG (10 ng/ml) and harvested at indicated time points. MRTF-A localization was examined by immunofluorescence staining. (B) LX-2 cells were treated with EREG (10 ng/ml) and harvested at indicated time points. MRTF-A localization was examined by cell fractionation followed by Western blotting. Quantification was performed with Image Pro based on three independent experiments. (C,D) LX-2 cells were transfected with siRNAs targeting MRTF-A or scrambled siRNA (SCR) treated with recombinant EREG (10 ng/ml) for 24 h. Gene expression was examined by qPCR and Western. Quantification was performed with Image Pro based on three independent experiments. (E,F) LX-2 cells were treated with recombinant EREG (10 ng/ml) in the presence or absence of CCG for 24 h. Gene expression was examined by qPCR and Western. Quantification was performed with Image Pro based on three independent experiments.