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. 2021 Jan 15;8:624985. doi: 10.3389/fcell.2020.624985

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Copyright © 2021 Yang, Liu, Chen, Ye, Wang and Zeng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overview of proteomic and phosphoproteomic analyses in granulosa cells obtained from ovaries with different follicular statuses. (A) Proteomic and phosphoproteomic analyses workflow in granulosa cells from healthy (H), slightly atretic (SA), and atretic (A) porcine follicle samples. (B) The distributions of raw MS/MS length of peptides and phosphopeptides quantified from proteomic and phosphoproteomic data, respectively. (C) Reproducibility analysis of the samples. (D) The amounts of DEPs in different comparable groups. (E) The amounts of the differentially quantified phosphosites and phosphoproteins in different comparable groups.