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. 2021 Jan 15;8:624985. doi: 10.3389/fcell.2020.624985

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Copyright © 2021 Yang, Liu, Chen, Ye, Wang and Zeng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expressions of caspase6, cleaved caspase6, p-laminA/C-S22, laminA/C, and cleaved laminA/C in GCs from healthy (H), slightly atretic (SA), and atretic (A) follicles, as well as the possible regulatory pathways of caspase6, caspase3, laminA/C, and apoptosis. (A) Protein levels of caspase6, cleaved caspase6, p-laminA/C-S22, laminA/C, and cleaved laminA/C in GCs from H, SA, and A follicles were detected by Western blotting. Quantitative analysis of these proteins is shown in histograms. The bars are labeled with completely different letters (a, b, c) indicating significant difference, P < 0.05. (B) The expression of laminA/C and caspase6 in GCs of follicles in different health states was detected by immunofluorescence. Nuclei were stained with Hoechst. Scale bar = 100 μm. TC, theca cell; GC, granulosa cell; BM, basement membrane. (C) Hypothetical model of the regulatory pathways of caspase6, caspase3, and laminA/C in GCs during follicular atresia. (D) Caspase6 S76A increased the ratios of caspase6 cleavage compared with the wild-type caspase6 and caspase6 S76D, after transfection and apoptosis induction. 293T cells were transfected with caspase6, caspase6 S76A, and caspase6 S76D plasmids for 16 h; the CN and p-EGFP-N1 are transfection control. Then, the cells were treated with 30 μg/ml CHX and 50 ng/ml TNFα for 12 h. The cell was harvested for Western blotting analysis. The ratios of cleaved caspase6/caspase6 and cleaved caspase3/caspase3 in the pig caspase6 (P), pig caspase6 S76A (PA), and pig caspase6 S76D (PD) groups were shown in the graph. The data are representative of three independent experiments. (E) Align the amino acid sequence near the Ser76 of caspase6 in human, rat, mouse, and pig. (F) Apoptotic rates of GFP positive cells after transfection with caspase6, caspase6 S76A, and caspase6 S76D plasmids. 293T cells were transfected and apoptosis-induced the same as described above and were analyzed by flow cytometry after Annexin V (PE) and 7-AAD (PerCP) staining. The data are representative of five independent experiments.