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. 2021 Jan 28;11:2504. doi: 10.1038/s41598-021-81815-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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SFN induce cell apoptosis in GC cells. (A) Increased apoptosis induction by SFN in BGC-823 and MGC-803 cells by Hoechst staining (× 200), the arrow indicates the apoptotic cells. The apoptosis rate in BGC-823 (B) and MGC-803 (C) cells treated with different concentration of SFN for 48 h was detected by flow cytometry after Annexin V/PI double staining. The apoptosis rate was calculated and depicted in a bar chart, and the values indicate the mean ± SD for three separate experiments, the statistical values were determined by Student’s t-test, *P < 0.05, **P < 0.01.